The Environmental-Endocrine Basis of Gynandromorphism (Intersex) in a Crustacean
Commensurate with the decline in many crustacean populations has been an accumulation in reports of sexually ambiguous individuals within these populations. The cause of gynandromorphism or intersex...
View ArticleA complete mitochondrial genome sequence of Asian black bear Sichuan...
We obtained the complete mitochondrial genome of U.thibetanus mupinensis by DNA sequencing based on the PCR fragments of 18 primers we designed. The results indicate that the mtDNA is 16 868 bp in...
View ArticleRNAi-based conditional gene knockdown in mice using a U6 promoter driven vector
RNA interference (RNAi) is a powerful tool widely used for studying gene function in a number of species. We have previously developed an approach that allows conditional expression of a polymerase...
View ArticleMouse 24p3 Protein Has an Effect on L929 Cell Viability
It is well known that mouse uterine 24p3 protein, is an acute phase protein, secreted from the L929 cell line, and that it will be induced by the dexamethasone stimulation of the cell. We investigated...
View ArticleEssential role of Elovl4 in very long chain fatty acid synthesis, skin...
Mutations in the gene ELOVL4 have been shown to cause stargardt-like macular dystrophy. ELOVL4 is part of a family of fatty acid elongases and is yet to have a specific elongase activity assigned to...
View ArticleDepletion of ceramides with very long chain fatty acids causes defective skin...
Very long chain fatty acids (VLCFA), either free or as components of glycerolipids and sphingolipids, are present in many organs. Elongation of very long chain fatty acids-4 (ELOVL4) belongs to a...
View ArticleSwine Genome Science Comes of Age
Pigs were among the first animals to be domesticated and pork is one of the most widely eaten meats in the world today. The pig has also been an excellent biomedical model for understanding a variety...
View ArticleAdvances in Swine Transcriptomics
The past five years have seen a tremendous rise in porcine transcriptomic data. Available porcine Expressed Sequence Tags (ESTs) have expanded greatly, with over 623,000 ESTs deposited in Genbank....
View ArticleGenetic Resources, Genome Mapping and Evolutionary Genomics of the Pig (Sus...
The pig, a representative of the artiodactyla clade, is one of the first animals domesticated, and has become an important agriculture animal as one of the major human nutritional sources of animal...
View ArticleCharacterizing Linkage Disequilibrium in Pig Populations
Knowledge of the extent and range of linkage disequilibrium (LD), defined as non-random association of alleles at two or more loci, in animal populations is extremely valuable in localizing genes...
View ArticleAdvances in Swine Biomedical Model Genomics
This review is a short update on the diversity of swine biomedical models and the importance of genomics in their continued development. The swine has been used as a major mammalian model for human...
View ArticleIdentifying the Future Needs for Long-Term USDA Efforts in Agricultural...
Agricultural animal research has been immensely successful over the past century in developing technology and methodologies that have dramatically enhanced production efficiency of the beef, dairy,...
View ArticleAdvances in QTL Mapping in Pigs
Over the past 15 years advances in the porcine genetic linkage map and discovery of useful candidate genes have led to valuable gene and trait information being discovered. Early use of exotic breed...
View ArticleOverexpression of Selenoprotein H Reduces Ht22 Neuronal Cell Death after UVB...
Selenoproteins have been shown to exhibit a variety of biological functions, including antioxidant functions, maintaining cellular redox balance, and heavy metal detoxification. UV irradiation-induced...
View ArticleStructural stability and endonuclease activity of a PI-SceI GFP-fusion protein
Homing endonucleases are site-specific and rare cutting endonucleases often encoded by intron or intein containing genes. They lead to the rapid spread of the genetic element that hosts them by a...
View ArticleCharacterization of transcriptional regulation of neurogranin by nitric oxide...
Neurogranin (Ng), a calmodulin (CaM)-binding protein kinase C (PKC) substrate, regulates the availability of Ca2+/CaM complex and modulates the homeostasis of intracellular calcium in neurons....
View ArticleMCEF is localized to the nucleus by protein sequences encoded within three...
Translocations between the human Mixed Lineage Leukemia (MLL) and AF4 Family (AFF) member genes, are implicated in leukemia. Mutations to AFFs can disrupt lymphopoesis, CNS development and...
View ArticleTyrosine Sulfation of Statherin
Tyrosylprotein sulfotransferase (TPST), responsible for the sulfation of a variety of secretory and membrane proteins, has been identified and characterized in submandibular salivary glands (William...
View ArticleSilencing of the Pink1 Gene Expression by Conditional RNAi Does Not Induce...
Transgenic RNAi, an alternative to the gene knockout approach, can induce hypomorphic phenotypes that resemble those of the gene knockout in mice. Conditional transgenic RNAi is an attractive choice...
View ArticleA polychromator-based microspectrophotometer
A microspectrophotometer is a digital microscope used to measure absorption and fluorescence spectra. In this paper we describe a polychromator-based microspectrophotometer that performs in vivo...
View ArticleEnrichment of coenzyme Q10 in plasma and blood cells: defense against...
Coenzyme Q10 (CoQ10) concentration in blood cells was analyzed by HPLC and compared to plasma concentration before, during, and after CoQ10 (3 mg/kg/day) supplementation to human probands. Lymphocyte...
View ArticleProteomics Analysis of the Expression of Neurogranin in Murine Neuroblastoma...
Neurogranin (Ng) is a neural-specific, calmodulin (CaM)-binding protein that is phosphorylated by protein kinase C (PKC). Although its biochemical property has been well characterized, the...
View ArticleDevelopment of a highly sensitive and selective microplate chemiluminescence...
A microplate chemiluminescence enzyme immunoassay (CLEIA) with high sensitivity, selectivity and reproducibility was developed for the determination of free thyroxine (FT4) in human serum. A...
View ArticleEffects of BRCA1 Transgene Expression on Murine Mammary Gland Development and...
To characterize the role of BRCA1 in mammary gland development and tumor suppression, a transgenic mouse model of BRCA1 overexpression was developed. Using the mouse mammary tumor virus (MMTV)...
View ArticleHuman Maf1 negatively regulates RNA Polymerase III transcription via the...
RNA polymerase III (RNA pol III) transcribes many of the small structural RNA molecules involved in processing and translation, thereby regulating the growth rate of a cell. Initiation of pol III...
View ArticleThe Forces Behind Cell Movement
Cell movement is a complex phenomenon primarily driven by the actin network beneath the cell membrane, and can be divided into three general components: protrusion of the leading edge of the cell,...
View ArticleIn search of a function for the most frequent naturally-occurring length...
Although the prototypical HIV-1 LTR sequences were determined 22 years ago from the initial isolate, elucidating which transcription factors are critical to replication in vivo, has been difficult....
View ArticleReduced Risk of Human Lung Cancer by Selective Cyclooxygenase 2 (Cox-2)...
We conducted a case control study of selective cyclooxygenase-2 (COX-2) blocking agents and lung cancer. A total of 492 newly diagnosed lung cancer cases were ascertained during January 1, 2002 to...
View ArticleThe responses of HT22 cells to the blockade of mitochondrial complexes and...
Mitochondria are the major reactive oxygen species (ROS) – generating sites in mammalian cells. Blockade of complexes in the electron transport chain (ETC) increases the leakage of single electrons to...
View ArticleDifferences in Apoptosis and Cell Cycle Distribution between Human Melanoma...
Introduction of human chromosome 6 into malignant melanoma cell line UACC903 resulted in generation of the chromosome 6-mediated suppressed cell subline UACC903(+6) that displays attenuated growth...
View ArticleSuperoxide Anion Radical Scavenging Activities of Herbs and Pastures in...
Free radicals are not only destructive to the living cells but also reduce the quality of animal products through oxidation. As a result the superoxide anion radical (O2・-), one of the most...
View ArticleThe Amphioxus SoxB Family: Implications for the Evolution of Vertebrate Placodes
Cranial placodes are regions of thickened ectoderm that give rise to sense organs and ganglia in the vertebrate head. Homologous structures are proposed to exist in urochordates, but have not been...
View ArticleAn mRNA-protein Fusion at N-terminus for Evolutionary Protein Engineering
A novel method to link a nascent protein (phenotype) to its mRNA (genotype) covalently through the N-terminus was developed. The mRNA harboring amber stop codon at just downstream of initiation site...
View ArticleHigh level glucose increases mutagenesis in human lymphoblastoid cells
Epidemiological data have suggested an increased cancer rates in diabetic patients, for which the underlying mechanism is poorly understood. We studied whether high level of glucose (HG) treatment...
View ArticleVersican Expression during Synovial Joint Morphogenesis
The extracellular matrix (ECM) plays a critical role in governing cell behavior and phenotype during limb skeletogenesis. Chondroitin sulfate proteoglycans (Cspgs) are highly expressed in the ECM of...
View ArticleReversal of Systemic Hypertension-Associated Cardiac Remodeling in Chronic...
Elevated oxidative stress has been characterized in numerous disorders including systemic hypertension, arterial stiffness, left ventricular hypertrophy (LVH) and heart failure. The peroxisome...
View ArticleThermal injury-plus-sepsis contributes to a substantial deletion of...
Thermal injury (TI) with septic complications continues to be a serious clinical problem. One of the main concerns in such patients is immunosuppression related to functional derangements in...
View ArticleDNA damage repair is unaffected by mimicked heterozygous levels of BRCA2 in...
Functional loss of both alleles of the breast cancer susceptibility gene, BRCA2, facilitates tumorigenesis. However, the direct effects of BRCA2 heterozygosity remain unclear. Here, BRCA2...
View ArticleCharles River Sprague Dawley Rats Lack Early Age-Dependent Susceptibility to...
Developmental stages of mammary glands influence their susceptibility to initiating events related to carcinogenesis. The “window of susceptibility” to mammary carcinogenesis is classically defined as...
View ArticleCandidate Gene Identification Approach: Progress and Challenges
Although it has been widely applied in identification of genes responsible for biomedically, economically, or even evolutionarily important complex and quantitative traits, traditional candidate gene...
View ArticlecDNA Cloning and Overexpression of Acidic Ribosomal Phosphoprotein P1 Gene...
RPLP1 is one of acidic ribosomal phosphoproteins encoded by RPLP1 gene, which plays an important role in the elongation step of protein synthesis. The cDNA of RPLP1 was cloned successfully for the...
View ArticlePhycobilisomes linker family in cyanobacterial genomes: divergence and evolution
Cyanobacteria are the oldest life form making important contributions to global CO2 fixation on the Earth. Phycobilisomes (PBSs) are the major light harvesting systems of most cyanobacteria species....
View ArticleImmunoregulatory role of intestinal surfactant-like particles during...
Surfactants like particles (SLP) are secreted by Intestinal epithelium. These particles have the ability to lower surface tension of intestinal epithelial cells and contain small amounts of surfactant...
View ArticleAdjuvant effect of anti-4-1BB mAb administration in adoptive T cell therapy...
Administration of anti-4-1BB mAb has been found to be a potent adjuvant when combined with other therapeutic approaches, e.g. chemotherapy, cytokine therapies, anti-OX40 therapy, and peptide or DC...
View ArticleRational Design of Analyte Channels of the Green Fluorescent Protein for...
A novel solvent-exposed analyte channel, generated by F165G substitution, on the surface of green fluorescent protein (designated His6GFPuv/F165G) was successfully discovered by the aid of molecular...
View ArticleThe Growth-hormone inducible transmembrane protein (Ghitm) belongs to the Bax...
The conserved protein domain UPF0005 is a protein family signature distributed among many species including fungi and bacteria. Although of unknown functionality this motif has been found in newly...
View ArticleSmad3 -signalling and Th2 cytokines in normal mouse airways and in a mouse...
This study investigates the role of Smad3 signalling for the T-helper2 (Th2) cytokine homeostasis in normal lungs and in a mouse model of asthma. We used mice deficient for Smad3, a central part of...
View ArticleConcerted Transcriptional Regulation by BRCA1 and COBRA1 in Breast Cancer Cells
Cofactor of BRCA1 (COBRA1) was first identified as a protein that binds to the breast cancer susceptibility gene product BRCA1. COBRA1 modulates estrogen-dependent and independent transcription and...
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